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Analysis of active substances and biological effects of some non-tradizional cereals
Pecháček, Michal ; Vysoká, Marie (referee) ; Márová, Ivana (advisor)
This thesis deals with the characterization of active substances and biological effects of non-traditional cereals. Basic substances of these non-traditional cereals such as carbohydrates or proteins are characterized in this work. Active compounds such as antioxidants, polyphenols or -glucans are also characterized. These substances were mainly analysed by spectrophotometric methods. The theoretical part describes cereals in general. It focuses on grain morphology, cereal products and mostly on chemical composition. The summary of substances appearing in the cereal grain such as carbohydrates, proteins, vitamins, minerals etc. was processed in this part. In the practical part, the samples of the non-traditional cereals such as amaranth, sorghum, millet, kamut, buckwheat, quinoa, Job’s tears and teff were analysed. Amaranth, millet and buckwheat were also analysed in the form of flakes. Teff was analysed only in the form of flakes. Sorghum and quinoa were analysed also in their coloured variations. Firstly, the water extracts were prepared, then they were used for determination of antioxidant activity, the content of polyphenols compounds and flavonoids. The hydrolysed samples were used for determination of the carbohydrates. For other basic analysis, samples were used in the form of powder. Best results were measured for quinoa, amaranth and buckwheat. Besides basic analysis, the content of -glucans was measured. However, the content of -glucans in these non-traditional cereals was very low. Selected cereals were tested for cytotoxicity on human cells. Cytotoxicity was evaluated by using the MTT cytotoxicity test on human keratinocytes HaCaT and human caucasian colon adenocarcinoma CaCO-2. The sensory analysis was carried out in the last part of the thesis. There were tested 6 samples. From these samples were made muffins that were analysed and sensorically evaluated.
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Radioactive Waste in Hospital Facilities
Bradáč, Antonín ; Houdková, Lucie (referee) ; Tabasová, Andrea (advisor)
The thesis is focused on a little-known issue of institutional waste produced by hospitals, the creation, collection and subsequent disposal. This thesis described the distribution of waste from various aspects, especially the characteristics and their subsequent use in the effective elimination. This thesis deals with not only this issue, but trying to get closer to negative impact of ionizing radiation on the human body, not only in the current level of radiation protection, but also in terms of the historical development of different sources and work with them, especially in connection with the use of ionizing radiation in normal human activity but also through the list of nuclear accidents. The practical part of this thesis compares the three types of hospitals primarily on the basis of a personal meeting with the heads of various departments involved in the management of institutional waste, and materials derived their visit. Specifically, the hospital Atlas and Tomas Bata Regional Hospital (both situated in Zlin) and specialized workplace Masaryk cancer institute in Brno.
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Systems for Generation of Pulse Magnetic Vector Potential
Hanák, Pavel ; Makáň,, Florian (referee) ; Bartušek, Karel (referee) ; Vrba, Kamil (advisor)
The doctoral thesis is focused on research, design, implementation and testing of systems for the application of magnetic vector potential to biological materials. The main objective was to analyze and design systems which could generate magnetic vector potential without the presence of other unwanted fields or at least amplify its intensity. Moreover, the systems designed had to eliminate other foreign effects on the biological samples, especially the influence of waste heat from the coils. Toroidal coils were employed to generate the vector potential, because they confine the unwanted magnetic induction inside their core thanks to their shape. The thesis employed coils with two different outer diameters, specifically 102 and 600 mm. To excite the coils, four current pulse generators capable of delivering currents of up to 100 A were constructed. The systems’ generated fields were comprehensively analyzed with the help of finite-element simulations in ANSYS. To simplify the design phase, analytical equations for the calculation of vector potential intensity at an arbitrary point around the toroidal coils were also derived. A method employing electromagnetic shielding made of two different materials was developed to suppress the unwanted fields. To eliminate the influence of heat, the 102 mm system employed air cooling and the 600 mm system employed a closed water loop to equalize the temperatures of biological samples. The biological effects of both systems were tested on genetically modified bio-luminescent bacteria Escherichia coli K12 luxABCDEamp. The thesis was created in connection with the research project of The Ministry of Education, Youth and Sports of the Czech Republic named “Research into the effect of a combination of substances for targeted therapy and inhibitory action of the field pulse vector magnetic potential on oncogenous diseases”, No. 2B08063.
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Constituents of Rosmarinus officinalis and their biological effects
Klížová, Aneta ; Karlíčková, Jana (advisor) ; Tůmová, Lenka (referee)
CHARLES UVIVERSITY THE FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACOGNOSY AND PHARMACEUTICAL BOTANY Title of the diploma thesis: CONSTITUENTS OF ROSEMARY AND THEIR BIOLOGICAL EFFECTS Candidate: Aneta Klížová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Diploma thesis 2021/2022, pp. 89 Rosemary (Rosmarinus officinalis L.) belongs to the Lamiaceae family. It is a bushy, evergreen perennial plant coming from the Mediterranean. Two pharmacopoeia herbal substances are used from this plant - leaves and essential oil. Rosemary has a wide range of uses. It is mainly used as a spice in the kitchen. Due to its antioxidant effects, it is used as a food preservative. It is also added to various cosmetic products such as soaps, creams, shampoos and perfumes. Rosemary and its components (carnosol, carnosic acid and rosmarinic acid and others) show many effects, which include analgesic, antioxidant, antiinflammatory, anticancer, antirheumatic, spasmolytic, antihepatotoxic, carminative, atherosclerotic, choleretic, antidepressant effects and others. It also works against bacteria and viruses. Rosemary is widely used as a traditional medicine, so it is included in the EMA monograph. Extract of rosemary or substances isolated from it have been tested in many in vitro and in vivo studies. Essential oil...
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Analysis of active substances and biological effects of some non-tradizional cereals
Pecháček, Michal ; Vysoká, Marie (referee) ; Márová, Ivana (advisor)
This thesis deals with the characterization of active substances and biological effects of non-traditional cereals. Basic substances of these non-traditional cereals such as carbohydrates or proteins are characterized in this work. Active compounds such as antioxidants, polyphenols or -glucans are also characterized. These substances were mainly analysed by spectrophotometric methods. The theoretical part describes cereals in general. It focuses on grain morphology, cereal products and mostly on chemical composition. The summary of substances appearing in the cereal grain such as carbohydrates, proteins, vitamins, minerals etc. was processed in this part. In the practical part, the samples of the non-traditional cereals such as amaranth, sorghum, millet, kamut, buckwheat, quinoa, Job’s tears and teff were analysed. Amaranth, millet and buckwheat were also analysed in the form of flakes. Teff was analysed only in the form of flakes. Sorghum and quinoa were analysed also in their coloured variations. Firstly, the water extracts were prepared, then they were used for determination of antioxidant activity, the content of polyphenols compounds and flavonoids. The hydrolysed samples were used for determination of the carbohydrates. For other basic analysis, samples were used in the form of powder. Best results were measured for quinoa, amaranth and buckwheat. Besides basic analysis, the content of -glucans was measured. However, the content of -glucans in these non-traditional cereals was very low. Selected cereals were tested for cytotoxicity on human cells. Cytotoxicity was evaluated by using the MTT cytotoxicity test on human keratinocytes HaCaT and human caucasian colon adenocarcinoma CaCO-2. The sensory analysis was carried out in the last part of the thesis. There were tested 6 samples. From these samples were made muffins that were analysed and sensorically evaluated.
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Systems for Generation of Pulse Magnetic Vector Potential
Hanák, Pavel ; Makáň,, Florian (referee) ; Bartušek, Karel (referee) ; Vrba, Kamil (advisor)
The doctoral thesis is focused on research, design, implementation and testing of systems for the application of magnetic vector potential to biological materials. The main objective was to analyze and design systems which could generate magnetic vector potential without the presence of other unwanted fields or at least amplify its intensity. Moreover, the systems designed had to eliminate other foreign effects on the biological samples, especially the influence of waste heat from the coils. Toroidal coils were employed to generate the vector potential, because they confine the unwanted magnetic induction inside their core thanks to their shape. The thesis employed coils with two different outer diameters, specifically 102 and 600 mm. To excite the coils, four current pulse generators capable of delivering currents of up to 100 A were constructed. The systems’ generated fields were comprehensively analyzed with the help of finite-element simulations in ANSYS. To simplify the design phase, analytical equations for the calculation of vector potential intensity at an arbitrary point around the toroidal coils were also derived. A method employing electromagnetic shielding made of two different materials was developed to suppress the unwanted fields. To eliminate the influence of heat, the 102 mm system employed air cooling and the 600 mm system employed a closed water loop to equalize the temperatures of biological samples. The biological effects of both systems were tested on genetically modified bio-luminescent bacteria Escherichia coli K12 luxABCDEamp. The thesis was created in connection with the research project of The Ministry of Education, Youth and Sports of the Czech Republic named “Research into the effect of a combination of substances for targeted therapy and inhibitory action of the field pulse vector magnetic potential on oncogenous diseases”, No. 2B08063.
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Radioactive Waste in Hospital Facilities
Bradáč, Antonín ; Houdková, Lucie (referee) ; Tabasová, Andrea (advisor)
The thesis is focused on a little-known issue of institutional waste produced by hospitals, the creation, collection and subsequent disposal. This thesis described the distribution of waste from various aspects, especially the characteristics and their subsequent use in the effective elimination. This thesis deals with not only this issue, but trying to get closer to negative impact of ionizing radiation on the human body, not only in the current level of radiation protection, but also in terms of the historical development of different sources and work with them, especially in connection with the use of ionizing radiation in normal human activity but also through the list of nuclear accidents. The practical part of this thesis compares the three types of hospitals primarily on the basis of a personal meeting with the heads of various departments involved in the management of institutional waste, and materials derived their visit. Specifically, the hospital Atlas and Tomas Bata Regional Hospital (both situated in Zlin) and specialized workplace Masaryk cancer institute in Brno.
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The use of the terapeutic laser in medicine
VESELÝ, Jan
Modern diagnostics and therapy is nowadays very strongly influenced by technological developments. New discoveries in physics, electronics and other fields lead to search for applications in the medical field. Laser is no exception. It is one of the most important technological discoveries of the late 20th century. A lot of targeted researches and multiple observations demonstrated beneficial effects of the laser radiation on living tissue. Laser was also confirmed to be used in medicine. Recently, laser therapy has been more and more popular. Lasers have found their place and application in almost all areas of medicine, both in treatment and also in research; it has been constantly developing dynamically. Laser Therapy (LLLT) is a branch of phototherapy, which uses light (laser or non-coherent monochromatic beam) for treatment of various diseases. Laser means light amplification by stimulated emission of radiation. This method of treatment is special as it does not lead to serious side effects and treatment does not take so much time to show effectiveness. Laser therapy is very far from practical use of surgical lasers. Laser Therapy (LLLT) is something different containing a lot of scientific utilization characteristics of the laser beam; it uses different colours of the beam to achieve the desired effect for treatment of a particular disease, the correct dosage etc. Surgery uses surgical properties of lasers. These include e.g. surgical knife cutting or tissue welding. Laser therapy possibilities, however, go much further. In this therapy laser is not a mere tool, but a medicine in itself. The power of the therapeutic laser power can be thousands of times smaller and weaker than the power of the surgical one. Many patients still assume that laser therapy consists in the use of surgical lasers, which is, however, not correct. Laser therapy uses non-surgical lasers, which neither cut nor burn. Application of therapeutic laser produces the desired effect in the patient's body by means of the way of cell changes in all cells that are exposed to the beam. It causes increased protein synthesis, immunocorrection, increased ATP production, increased enzymatic activity and circulation, and collagen synthesis. Lasers also regulate lipid metabolism, normalize blood pressure, reduce swelling and inflammation, and reduce pain. Furthermore, leasers have an anti-inflammatory effect, they cause increased cell division and increase immunity against diseases. Lasers have stimulatory, and generally rejuvenilizing and generally healing effects, but when adjusting their dosage they are inhibitory. Thus, using the therapeutic laser brings effective treatment, even if allopathic approach calls many of them incurable.
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